DLD Protein (AA 36-509) (His tag)
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Quick Overview for DLD Protein (AA 36-509) (His tag) (ABIN666962)
抗原
See all DLD 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 36-509
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标记
- This DLD protein is labelled with His tag.
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序列
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MADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F -
产品特性
- DLD, 36-509aa, Human, His tag, E.coli
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纯化方法
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 200unit/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.
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Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) protein (GST tag)
DLD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dihydrolipoamide Dehydrogenase (DLD) proteinDLD 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Dihydrolipoamide Dehydrogenase (DLD) protein (Myc-DYKDDDDK Tag)DLD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 0.1M NaCl, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DLD (Dihydrolipoamide Dehydrogenase (DLD))
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别名
- Dihydrolipoamide Dehydrogenase/DLD
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背景
- DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSuD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 54.4 kDa (511aa)
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NCBI登录号
- NP_000099
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途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
抗原
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