Quick Overview for ENO1 Protein (AA 1-434) (ABIN666899)
抗原
See all ENO1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-434
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序列
- MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
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产品特性
- Alpha-enolase, 1-434aa, Human, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
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Enolase 1 (ENO1) (AA 1-434) protein (GST tag)
ENO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) protein (His tag)ENO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Enolase 1 (ENO1) (AA 2-433) protein (His-IF2DI Tag)ENO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Enolase 1 (ENO1) (AA 325-434) protein (GST tag)ENO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) (AA 1-434) (Active) protein (His tag)ENO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, EAA Active
Enolase 1 (ENO1) (AA 1-434) (Active) proteinENO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS Active
Enolase 1 (ENO1) (AA 2-428), (partial) protein (GST tag)ENO1 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Enolase 1 (ENO1) (AA 2-433), (partial) protein (His tag)ENO1 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM MgSO4, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ENO1 (Enolase 1 (ENO1))
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别名
- Enolase 1/ENO1
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背景
- Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.
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分子量
- 47.1 kDa (434aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001419
抗原
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