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Quick Overview for GFP Protein (AA 1-238) (ABIN666762)
抗原
See all GFP 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-238
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序列
- MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
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产品特性
- GFP, 1-238 aa, Aequorea victoria, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Green Fluorescent Protein (GFP) protein (His tag)
GFP 宿主: Aequorea victoria 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by SDS-PAGE
Green Fluorescent Protein (GFP) (AA 1-238) protein (His tag)GFP 宿主: Aequorea victoria 宿主: 酵母菌 Recombinant > 95 % SDS, WB, IP, ELISA
Green Fluorescent Protein (GFP) proteinGFP 宿主: Aequorea victoria 宿主: 大肠杆菌（E. Coli） Recombinant Ct, IA
Green Fluorescent Protein (GFP) (AA 1-238), (full length) protein (His-SUMO Tag)GFP 宿主: Aequorea victoria 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Green Fluorescent Protein (GFP) (AA 1-238), (full length) protein (His tag)GFP 宿主: Aequorea victoria 宿主: 酵母菌 Recombinant > 90 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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注意事项
- Avoid repeated freezing and thawing cycles
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.
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分子量
- 26.8 kDa (238aa) confirmed by MALDI-TOF
抗原
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