< 1 EU per 1ug of protein (determined by LAL method)
Biological Activity Comment
Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.
ALDH1A1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 90 %
SDS, EAA
Active
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM DTT, 1 mM EDTA, 10 % glycerol
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
抗原
ALDH1A1
(Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
别名
Aldehyde dehydrogenase 2/ALDH2
背景
ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.