Quick Overview for COMT Protein (AA 51-271) (ABIN666744)
抗原
See all COMT 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 51-271
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序列
- MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P
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产品特性
- COMT, 51-271 aa, Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Catechol-O-Methyltransferase (COMT) (AA 1-271) protein (His tag)
custom-made COMT 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Catechol-O-Methyltransferase (COMT) (AA 1-265) protein (His tag)custom-made COMT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Catechol-O-Methyltransferase (COMT) (AA 51-271) protein (His-IF2DI Tag)COMT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Catechol-O-Methyltransferase (COMT) (AA 1-182) protein (GST tag)COMT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM MgCl2, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- COMT (Catechol-O-Methyltransferase (COMT))
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别名
- COMT
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背景
- Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 24.4 kDa (221aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000745
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途径
- Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome
抗原
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