Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) 蛋白
This Recombinant Aldo-Keto Reductase Family 1 Member B10-Like 1 protein is expressed in 大肠杆菌（E. Coli）.
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产品编号 ABIN666726
发货至:
中国
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Quick Overview for Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) 蛋白 (ABIN666726)
抗原
Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Enzyme Activity Assay (EAA), SDS-PAGE (SDS)
纯度
> 95 %
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产品特性
- AA 1-316
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序列
- MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
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产品特性
- Aldose reductase, 1-316aa, Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
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Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) protein (His tag)
AKR1B10L1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % EAA, SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)
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别名
- Aldo-keto reductase 1B1/AKR1B1
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背景
- Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 35.8 kDa (316aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001619
抗原
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