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Quick Overview for PPIH Protein (AA 1-177) (ABIN666713)
抗原
See all PPIH 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-177
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序列
- MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
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产品特性
- Cyclophilin H(PPIH), 1-177aa, Human,Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 220nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.
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Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) (AA 1-177) protein (GST tag)
PPIH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) (AA 1-177) (Active) proteinPPIH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS Active
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) protein (Myc-DYKDDDDK Tag)PPIH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))
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别名
- Cyclophilin H/PPIH
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背景
- Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human u4/u6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the u4/u6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.
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分子量
- 19.2 kDa (177aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_006338
抗原
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