ENO2/NSE Protein (AA 1-434)
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北京 101111
Quick Overview for ENO2/NSE Protein (AA 1-434) (ABIN666709)
抗原
See all ENO2/NSE (ENO2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-434
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序列
- MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
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产品特性
- Neuron-Specific Enolase (NSE), 1-434aa, Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
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Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) protein (GST tag)
ENO2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) protein (His-IF2DI Tag)ENO2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) (Active) protein (His tag)ENO2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, EAA Active
Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) (Active) proteinENO2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5) containing 0.1 M KCl, 5 mM MgSO4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- Enolase 2/ENO2
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背景
- Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E. coli and purified by conventional chromatography techniques.
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分子量
- 47.2 kDa ( 434aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001966
抗原
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