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Quick Overview for PPIB Protein (AA 26-216) (ABIN666694)
抗原
See all PPIB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 26-216
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序列
- MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
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产品特性
- Cyclophilin B, 26- 216aa , Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 1,500nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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Cyclophilin B (PPIB) (AA 34-216) protein (GST tag)
PPIB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cyclophilin B (PPIB) (AA 34-216) protein (His tag)PPIB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cyclophilin B (PPIB) (AA 1-216) protein (GST tag)PPIB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Cyclophilin B (PPIB) (AA 34-216) (Active) protein (His tag)PPIB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, EAA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PPIB (Cyclophilin B (PPIB))
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别名
- Cyclophilin B/PPIB
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背景
- Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques
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分子量
- 21.2 kDa (192aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000933
抗原
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