G-CSF Protein (AA 31-204)
Our Local Distributor
北京 101111
Quick Overview for G-CSF Protein (AA 31-204) (ABIN666680)
抗原
See all G-CSF (CSF3) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 31-204
-
序列
- MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
-
产品特性
- G-CSF, Human, Recombinant, E.coli
-
纯化方法
- > 95% by SDS-PAGE
-
内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
-
Biological Activity Comment
- Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3ng/ml.
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
-
别名
- G-CSF
-
背景
- Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
-
分子量
- 18.8 kDa (175aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_757373
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
-