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Quick Overview for SETD7 Protein (AA 1-366) (ABIN666678)
抗原
See all SETD7 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-366
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序列
- MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK
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产品特性
- SET7/9 Histone methyltransferase Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (AA 1-366) protein (His tag)
custom-made SETD7 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (AA 1-366) protein (His tag)custom-made SETD7 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) (AA 110-366) protein (His-IF2DI Tag)SETD7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
SET Domain Containing (Lysine Methyltransferase) 7 (SETD7) protein (Myc-DYKDDDDK Tag)SETD7 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 50 mM Tris-HCl buffer ( pH 7.5) containing 0.2M NaCl, 5 mM DTT, 20 % glycerol
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注意事项
- Avoid repeated freezing and thawing cycles
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SETD7 (SET Domain Containing (Lysine Methyltransferase) 7 (SETD7))
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别名
- SETD7
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背景
- SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET 7/9, unlike most other SET proteins, is exclusively a mono-methylase, Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
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分子量
- 40.7 kDa (366aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_085151
抗原
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