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Streptavidin Protein (AA 25-183) (His tag)

This Recombinant Streptavidin protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.

SDS-PAGE (SDS) image for Streptavidin (AA 25-183) protein (His tag) (ABIN666648) (Streptavidin Protein (AA 25-183) (His tag))

1 image

(1 reference)

产品编号 ABIN666648

发货至: 中国

Quick Overview for Streptavidin Protein (AA 25-183) (His tag) (ABIN666648)

抗原

See all Streptavidin 蛋白

Streptavidin

蛋白类型

Recombinant

宿主

Streptomyces

资源

大肠杆菌（E. Coli）

应用范围

SDS-PAGE (SDS)

纯度

> 95 %

Discover our top product Streptavidin 蛋白

产品特性

AA 25-183

标记

This Streptavidin protein is labelled with His tag.

序列

DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ

产品特性

His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli

纯化方法

> 95% by SDS-PAGE
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Streptavidin (AA 37-159) protein (His tag)
宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS

Streptavidin protein (TRITC)
宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native FM, FLISA, IHC, FISH

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宿主: Streptomyces avidinii FACS, CA, MA

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宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, FM, FLISA, DB, IF

Streptavidin protein
宿主: Streptomyces avidinii 宿主: 大肠杆菌（E. Coli）

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

20 mM Tris-HCl buffer ( pH 7.5)

储存条件

4 °C,-20 °C,-80 °C

储存方法

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Lanz, Masi, Porciello, Cohnen, Cipria, Prakaash, Bálint, Raggiaschi, Galgano, Cole, Lepore, Dushek, Dustin, Sansom, Kalli, Acuto: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).
抗原

Streptavidin

背景

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.

分子量

17.5 kDa (167aa) confirmed by MALDI-TOF

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