His-Streptavidin (AA 25-183) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.
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(1 reference)Quick Overview for His-Streptavidin (AA 25-183) protein (His tag) (ABIN666648)
抗原
His-Streptavidin
蛋白类型
Recombinant
宿主
Streptomyces avidinii
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 % by SDS - PAGE
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产品特性
- AA 25-183
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标记
- His tag
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产品特性
- His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml (determined by Bradford assay)
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缓冲液
- Liquid in 20mM Tris pH 7.5
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储存条件
- 4 °C
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: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).
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: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." in: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).
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- His-Streptavidin
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背景
- Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2. NCBI no.: CAA27265, P22629
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分子量
- 17 kDa (167 aa), confirmed by MALDI-TOF.
抗原
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