Prolactin Protein (PRL) (AA 30-228)
Quick Overview for Prolactin Protein (PRL) (AA 30-228) (ABIN7676781)
抗原
See all Prolactin (PRL) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 30-228
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序列
- MQPLPICSAG DCQTSLRELF DRVVILSHYI HTLYTDMFIE FDKQYVQDRE FMVKVINDCP TSSLATPEDK EQALKVPPEV LLNLILSLVQ SSSDPLFQLI TGVGGIQEAP EYILSRAKEI EEQNKQLLEG VEKIISQAYP EAKGNGIYFV WSQLPSLQGV DEESKILSLR NTIRCLRRDS HKVDNFLKVL RCQIAHQNNC
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using Nb2-11 rat lymphoma cells. The ED50 range ≤3ng/ml.
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Prolactin (PRL) (Active) protein
PRL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Prolactin (PRL) protein (His tag)PRL 宿主: 绵羊 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Prolactin (PRL) protein (His tag)PRL 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Prolactin (PRL) (AA 30-226) protein (His-IF2DI Tag)PRL 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Prolactin (PRL) (AA 30-226) protein (His-IF2DI Tag)PRL 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Prolactin (PRL) (AA 29-227) protein (GST tag)PRL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Prolactin (PRL) (AA 29-227) protein (Fc Tag)PRL 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Prolactin (PRL) (AA 29-227) protein (Fc Tag,AVI tag,Biotin)PRL 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate-Buffered Saline ( pH 7.4)
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Prolactin (PRL)
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别名
- Prolactin
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背景
- Prolactin, also known as prl, is a hormone synthesized and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation). Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immuno-regulation. Recombinant mouse Prolactin, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 22.7 kDa (200aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_035294
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途径
- JAK/STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
抗原
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