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GST Protein (AA 1-218)

This anti-GST antibody (ABIN6388215) is a Escherichia coli (E. coli) antibody detecting GST in SDS, EAA. Suitable for Schistosoma japonicum.
产品编号 ABIN6388215
发货至: 中国

Quick Overview for GST Protein (AA 1-218) (ABIN6388215)

抗原

See all GST 蛋白
GST (Glutathione S Transferase (GST))

蛋白类型

Recombinant

生物活性

Active

宿主

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Schistosoma japonicum

资源

  • 9
  • 8
大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

纯度

> 90% by SDS-PAGE
  • 产品特性

    AA 1-218

    序列

    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

    内毒素水平

    < 1 EU per 1ug of protein (determined by LAL method)

    Biological Activity Comment

    Specific activity is > 30unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Bioactivity Validated

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid. In Phosphate Buffer Saline containing 10 % glycerol

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
  • 抗原

    GST (Glutathione S Transferase (GST))

    别名

    Glutathione S-transferase/GST

    背景

    GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST was expressed in E. coli and purified by conventional chromatography techniques.

    分子量

    25.4 kDa (218aa)

    UniProt

    P08515
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