Adenylate Kinase 2 Protein (AK2) (AA 1-239) (His tag)
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Quick Overview for Adenylate Kinase 2 Protein (AK2) (AA 1-239) (His tag) (ABIN5855039)
抗原
See all Adenylate Kinase 2 (AK2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-239
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标记
- This Adenylate Kinase 2 protein is labelled with His tag.
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序列
- MGSSHHHHHH SSGLVPRGSH MGSHMAPNVL ASEPEIPKGI RAVLLGPPGA GKGTQAPKLA ENFCVCHLAT GDMLRAMVAS GSELGKKLKA TMDAGKLVSD EMVVELIEKN LETPSCKNGF LLDGFPRTVR QAEMLDDLME KRKEKLDSVI EFSIQDSLLI RRITGRLIHP KSGRSYHEEF NPPKEPMKDD ITGEPLIRRS DDNEKALKTR LEAYHTQTTP LVEYYRKRGI HCAIDASQTP DIVFASILAA FSKATCKDLV MFI
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纯化方法
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 40unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.5) containing 10 % glycerol, 1 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Adenylate Kinase 2 (AK2)
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别名
- AK2
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背景
- Ak2, also known as Adenylate kinase 2 mitochondrial isoform a, is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Ak2 is localized in the mitochondrial intermembrane space and may pay a role in apoptosis. Recombinant mouse Ak2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 29.0 kDa (263aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001029138
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途径
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
抗原
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