Quick Overview for PTH Protein (AA 32-115) (ABIN6387772)
抗原
See all PTH 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 32-115
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序列
- MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50ng/ml.
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Parathyroid Hormone (PTH) (Active) protein
PTH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Parathyroid Hormone (PTH) (AA 32-115) protein (Fc Tag)PTH 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)PTH 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
Parathyroid Hormone (PTH) (AA 32-115) protein (His-IF2DI Tag)PTH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parathyroid Hormone (PTH) (AA 1-115) protein (GST tag)PTH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Parathyroid Hormone (PTH) (AA 32-115) protein (GST tag)PTH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In phosphate-buffered Saline( pH 7.4) containing 2 mM EDTA
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- PTH (Parathyroid Hormone (PTH))
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别名
- PTH
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物质类
- Hormone
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背景
- Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
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分子量
- 9.55 kDa (85aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000306
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UniProt
- P01270
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途径
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
抗原
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