AKR1C4 Protein (AA 1-323)
Quick Overview for AKR1C4 Protein (AA 1-323) (ABIN6387734)
抗原
See all AKR1C4 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-323
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序列
- MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
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Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (His tag)
custom-made AKR1C4 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-343) protein (His tag)AKR1C4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (GST tag)AKR1C4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) protein (Myc-DYKDDDDK Tag)AKR1C4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer( pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
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别名
- Aldo-keto Reductase 1C4/AKR1C4
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背景
- AKR1C1 also known as Aldo-keto reductase family 1 member C4, belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein was expressed in E. coli and purified by using conventional chromatography.
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分子量
- 37 kDa (323aa)
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NCBI登录号
- NP_001809
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UniProt
- P17516
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途径
- Steroid Hormone Biosynthesis
抗原
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