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AKR1C1 Protein (AA 1-323)

DDH 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % by SDS - PAGE SDS Active
产品编号 ABIN6387732
发货至: 中国
  • 抗原 See all AKR1C1 (DDH) 蛋白
    AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    AA 1-323
    宿主
    • 6
    • 1
    • 1
    资源
    • 2
    • 2
    • 2
    • 1
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS)
    序列
    MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
    纯度
    > 95 % by SDS - PAGE
    内毒素水平
    < 1.0 EU per 1ug of protein (determined by LAL method)
    Biological Activity Comment
    Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
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    Discover our top product DDH 蛋白
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    Bioactivity Validated

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Liquid. In 20  mM Tris-HCl buffer (  pH 8.5) containing 0.1M NaCl, 20 % glycerol
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • 抗原
    AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
    别名
    Aldo-keto reductase family 1 member C1 (DDH 产品)
    别名
    2-ALPHA-HSD Protein, 20-ALPHA-HSD Protein, C9 Protein, DD1 Protein, DD1/DD2 Protein, DDH Protein, DDH1 Protein, H-37 Protein, HAKRC Protein, HBAB Protein, MBAB Protein, aldo-keto reductase family 1 member C1 Protein, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) Protein, AKR1C1 Protein
    背景
    AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
    分子量
    36.7kDa (323aa)
    NCBI登录号
    NP_001344
    UniProt
    Q04828
    途径
    Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
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