Quick Overview for NME2 Protein (AA 1-152) (ABIN6387709)
抗原
See all NME2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-152
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序列
- MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
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Biological Activity Comment
- Specific activity is > 1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
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Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)
custom-made NME2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)custom-made NME2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (GST tag)NME2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) proteinNME2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
Nucleoside diphosphate kinase B (NME2) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)NME2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- NME2 (Nucleoside diphosphate kinase B (NME2))
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别名
- NM23-H2/NME2
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背景
- NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 17.2 kDa (152aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_001018149
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UniProt
- P22392
抗原
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