NAD Synthetase protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for NAD Synthetase protein (His tag) (ABIN6253505)
抗原
NAD Synthetase
蛋白类型
Recombinant
宿主
Mycobacterium tuberculosis
资源
大肠杆菌（E. Coli）
纯度
>98 % (SDS-PAGE)
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标记
- His tag
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原理
- NAD Synthetase (M. tuberculosis) (rec.) (His)
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特异性
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
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产品特性
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
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Biological Activity Comment
- ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- 0.5 mg/mL (Lot specific)
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浓度
- Lot specific
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缓冲液
- In 20 mM TRIS-HCl, pH 7.8.
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注意事项
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
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储存条件
- -20 °C,-80 °C
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储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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有效期
- 6 months
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- NAD Synthetase
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背景
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NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
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分子量
- ~75.0kDa
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UniProt
- P0A5L6
抗原
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