CXCL2 Protein (AA 32-100) (His tag,GST tag)
Quick Overview for CXCL2 Protein (AA 32-100) (His tag,GST tag) (ABIN6239880)
抗原
See all CXCL2 蛋白生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 32-100
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标记
- This CXCL2 protein is labelled with His tag,GST tag.
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产品特性
- Tag location: N-terminal His and GST Tag
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Biological Activity Comment
- Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GROb with DPP4.
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Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) protein
CXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) proteinCXCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) proteinCXCL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 1-107) protein (GST tag)CXCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 35-107) protein (His tag)CXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 32-100) protein (His tag)CXCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 28-100) protein (His tag)CXCL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) proteinCXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 35-107) protein (His-DYKDDDDK-Strep II Tag)CXCL2 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 28-100) protein (His tag)CXCL2 宿主: 小鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % SDS, WB, ELISA, IP
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应用备注
- Isoelectric Point: 6.7
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
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别名
- Growth Regulated Oncogene Beta (GROb)
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背景
- Alternative Names: CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2
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分子量
- 41kDa
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UniProt
- P30348
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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