DGKE Protein (AA 243-502) (His tag)
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北京 101111
Quick Overview for DGKE Protein (AA 243-502) (His tag) (ABIN6239838)
抗原
See all DGKE 蛋白生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 243-502
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标记
- This DGKE protein is labelled with His tag.
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产品特性
- Tag location: N-terminal His Tag
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Biological Activity Comment
- DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of DGKe with PDHA.
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Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-567) protein (His tag)
custom-made DGKE 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-564) protein (His tag)custom-made DGKE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-177) protein (GST tag)DGKE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Diacylglycerol Kinase, epsilon 64kDa (DGKE) protein (Myc-DYKDDDDK Tag)DGKE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Isoelectric Point: 7.7
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
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别名
- Diacylglycerol Kinase Epsilon (DGKe)
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背景
- Alternative Names: DAGK5, DGK, Diglyceride kinase epsilon
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分子量
- 33kDa
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UniProt
- P52429
抗原
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