DGKE Protein (AA 243-502) (His tag)
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- 抗原 See all DGKE 蛋白
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
- 生物活性
- Active
- 产品特性
- AA 243-502
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DGKE protein is labelled with His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of DGKe with PDHA.
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Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-177) protein (GST tag)
DGKE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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Diacylglycerol Kinase, epsilon 64kDa (DGKE) protein (Myc-DYKDDDDK Tag)DGKE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 7.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
- 别名
- Diacylglycerol Kinase Epsilon (DGKe) (DGKE 产品)
- 别名
- MGC81643 Protein, DGKE Protein, C87606 Protein, DAGK6 Protein, DGK Protein, DAGK5 Protein, NPHS7 Protein, diacylglycerol kinase epsilon S homeolog Protein, diacylglycerol kinase epsilon Protein, diacylglycerol kinase, epsilon Protein, dgke.S Protein, DGKE Protein, dgke Protein, LOC5575244 Protein, Dgke Protein
- 背景
- Alternative Names: DAGK5, DGK, Diglyceride kinase epsilon
- 分子量
- 33kDa
- UniProt
- P52429
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