LMYC Protein (AA 184-454) (His tag,GST tag)
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Quick Overview for LMYC Protein (AA 184-454) (His tag,GST tag) (ABIN6239821)
抗原
See all LMYC (MYCL) 蛋白生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 184-454
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标记
- This LMYC protein is labelled with His tag,GST tag.
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产品特性
- Tag location: N-terminal His and GST Tag
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Biological Activity Comment
- MYC (Myc proto-oncogene protein) is a nuclear phosphoprotein that binds specific sequence of DNA. MYC functions as a transcription factor and regulates transcription of target genes. It has been proven that c-Myc protein is intracellularly associated with TBP (TATA-binding protein) of the TFIID transcription initiation complex; besides, TRRAP (Transformation/transcription domain-associated protein) is thought to be an essential cofactor for the MYC. Thus a binding ELISA assay was conducted to detect the interaction of MYC with TBP and TRRAP. Briefly, recombinant human MYC were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TBP-coated and TRRAP microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MYC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MYC with TBP and TRRAP was shown in Figure 1 and Figure 2 separately, and this effect was in a dose dependent manner The binding activity of MYC with TBP. Figure 2. The binding activity of MYC with TRRAP
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Protein L-Myc (MYCL) (AA 1-368) protein (His tag)
custom-made MYCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein L-Myc (MYCL) (AA 1-364) protein (His tag)custom-made MYCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein L-Myc (MYCL) (AA 1-206) protein (GST tag)MYCL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein L-Myc (MYCL) proteinMYCL 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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应用备注
- Isoelectric Point: 6.6
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- LMYC (MYCL) (Protein L-Myc (MYCL))
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别名
- V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
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背景
- Alternative Names: C-Myc, BHLHE39, Myc Proto-Oncogene Protein, Class E basic helix-loop-helix protein 39, Transcription factor p64, Proto-oncogene c-Myc
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分子量
- 65kDa
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UniProt
- P01106
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途径
- Warburg Effect
抗原
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