Malate Dehydrogenase (MDH) 蛋白
This Recombinant Malate Dehydrogenase protein is expressed in not_set.
产品编号 ABIN621154
发货至:
中国
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Quick Overview for Malate Dehydrogenase (MDH) 蛋白 (ABIN621154)
抗原
See all Malate Dehydrogenase (MDH) 蛋白
Malate Dehydrogenase (MDH)
蛋白类型
Recombinant
宿主
小鸡
宿主
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纯度
> 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Unit Definition One unit is defined as 1 µmol of NAD + production per minute under the assay conditions (25°C, pH 7.5). Specific Activity Specific Activity > 710U/mg protein.
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产品特性
- Recombinant Malate Dehydrogenase
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Malate Dehydrogenase (MDH) (AA 2-312) protein (His tag)
MDH 宿主: Bacillus subtilis 宿主: 酵母菌 Recombinant > 90 % ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Each mg of protein contains 0.59mg NaPO 4 .It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18M omega cm H 2 O.
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储存条件
- -20 °C
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- Malate Dehydrogenase (MDH)
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别名
- Malate Dehydrogenase
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背景
- The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart. The MDH is purified by proprietary chromatographic techniques. Introduction: Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of pyruvate using NADPH. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate. Synonyms: Malate dehydrogenase cytoplasmic, EC 1.1.1.37, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375.
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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