IgE Protein (His tag)
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北京 101111
Quick Overview for IgE Protein (His tag) (ABIN6120361)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This IgE protein is labelled with His tag.
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原理
- Pig Immunoglobulin Heavy Constant Epsilon (IGHE) Protein
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序列
- SIQGPLVYPL TSCCKTGATS KTLGCLVRGY FPEPVTVTWD TGSLNSSTLT FPAVQDSSSS LYTVTSQVTI LGEWTNQKLT CSVAHAANTT IRTITGCTKN FTDPSLRFFY SSCDPHGDAQ ATIHLRCYIS GYTPGKMKVT WLVDGQEDRN LFSYTAPDQL EGKLASTYSE VNITQGQWAS QITYTCQVSY YGFIYEKHAL RCTAESEPRG VSAYLSPPTP LDLYVHKSPK LTCLVVDLAS SENVNLLWSR ENKGGVILPP PGPPVIKPQF NGTFSATSTL PVNVSDWIEG ETYYCNVTHP DLPKPILRSI SKGPGKRVTP EVYVLWSPDE LKKGRLTLTC LIQNFFPADI SVLWLRNDAP VQADRHSTTR PHKASDSLPS FFVYSRLVVS QSDWEQNKFA CEVIHEALPG SRTLQKEVSK NPGK
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Biological Activity Comment
- Not tested
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IgE (AA 209-428) protein (His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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缓冲液
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose.
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储存液
- Dithiothreitol (DTT), Other preservative
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- IgE
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别名
- Immunoglobulin E
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物质类
- Antibody
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分子量
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Calculated MW: 50.5 kDa
Observed MW (SDS-PAGE): 50 kDa -
UniProt
- K7ZJQ0
抗原
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