DDT Protein (AA 21-248) (His tag)
Quick Overview for DDT Protein (AA 21-248) (His tag) (ABIN5855098)
抗原
See all DDT 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 21-248
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标记
- This DDT protein is labelled with His tag.
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序列
- MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L
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D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)
DDT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT 宿主: 犬 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His tag)DDT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His tag)DDT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (GST tag)DDT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- DDT (D-Dopachrome Tautomerase (DDT))
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别名
- D-Dopachrome Decarboxylase
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背景
- Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihyroxyindole(DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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分子量
- 15.5 kDa (141aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_034157
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UniProt
- O35215
抗原
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