DDT Protein (AA 1-118) (His tag)
Our Local Distributor
北京 101111
Quick Overview for DDT Protein (AA 1-118) (His tag) (ABIN5855098)
抗原
See all DDT 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-118
-
标记
- This DDT protein is labelled with His tag.
-
序列
- MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L
-
纯化方法
- > 95% by SDS-PAGE
-
-
D-Dopachrome Tautomerase (DDT) (AA 2-118) (Active) protein (His tag)
DDT 宿主: 犬 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His tag)DDT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (His-IF2DI Tag)DDT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
D-Dopachrome Tautomerase (DDT) (AA 1-118) protein (GST tag)DDT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
D-Dopachrome Tautomerase (DDT) (AA 2-118) protein (His tag)DDT 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % SDS
D-Dopachrome Tautomerase (DDT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DDT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- DDT (D-Dopachrome Tautomerase (DDT))
-
别名
- DDT
-
背景
- Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5, 6-dihyroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
-
分子量
- 15.5 kDa (141aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_034157
抗原
-
