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GldA (AA 1-367) protein (His tag)

This Recombinant GldA protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN5853893
发货至: 中国
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Quick Overview for GldA (AA 1-367) protein (His tag) (ABIN5853893)

抗原

GldA

蛋白类型

Recombinant

宿主

E. coli

资源

  • 2
大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS)

纯度

> 95 %
  • 产品特性

    AA 1-367

    标记

    His tag

    序列

    MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE

    纯化方法

    > 95% by SDS-PAGE
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate-Buffered Saline ( pH 7.4) 10 % glycerol

    注意事项

    Avoid repeated freezing and thawing cycles

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • 抗原

    GldA

    背景

    GldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E. coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i. e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1, 2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1, 2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

    分子量

    41.1 kDa (390aa) confirmed by MALDI-TOF

    NCBI登录号

    NP_418380
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