Streptavidin Protein (AA 37-159) (His tag)
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Quick Overview for Streptavidin Protein (AA 37-159) (His tag) (ABIN5853015)
抗原
See all Streptavidin 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 37-159
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标记
- This Streptavidin protein is labelled with His tag.
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序列
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AEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP -
纯化方法
- > 90% by SDS-PAGE
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Streptavidin protein (HRP)
宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, ELISA, FM, IHC, DB, IF, IEM
Streptavidin protein (PE)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native ELISA, FM, FACS, MA, DB, IF, BCA, Func
Streptavidin (AA 25-183) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Streptavidin protein (TRITC)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native FM, FLISA, IHC, FISH
Streptavidin protein (Atto 425)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, FM, FLISA, DB, IF
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Streptavidin
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背景
- Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 15.6 kDa (148aa) confirmed by MALDI-TOF
抗原
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