CAPSD (AA 228-781) protein (His tag)
Quick Overview for CAPSD (AA 228-781) protein (His tag) (ABIN5711083)
抗原
蛋白类型
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 228-781
-
标记
- His tag
-
序列
- MTSVNSAEAS TGAGGGGSNS VKSMWSEGAT FSANSVTCTF SRQFLIPYDP EHHYKVFSPA ASSCHNASGK EAKVCTISPI MGYSTPWRYL DFNALNLFFS PLEFQHLIEN YGSIAPDALT VTISEIAVKD VTDKTGGGVQ VTDSTTGRLC MLVDHEYKYP YVLGQGQDTL APELPIWVYF PPQYAYLTVG DVNTQGISGD SKKLASEESA FYVLEHSSFQ LLGTGGTASM SYKFPPVPPE NLEGCSQHFY EMYNPLYGSR LGVPDTLGGD PKFRSLTHED HAIQPQNFMP GPLVNSVSTK EGDSSNTGAG KALTGLSTGT SQNTRISLRP GPVSQPYHHW DTDKYVTGIN AISHGQTTYG NAEDKEYQQG VGRFPNEKEQ LKQLQGLNMH TYFPNKGTQQ YTDQIERPLM VGSVWNRRAL HYESQLWSKI PNLDDSFKTQ FAALGGWGLH QPPPQIFLKI LPQSGPIGGI KSMGITTLVQ YAVGIMTVTM TFKLGPRKAT GRWNPQPGVY PPHAAGHLPY VLYDPTATDA KQHHRHGYEK PEELWTAKSR VHPL
-
纯化方法
- SDS-PAGE
-
-
CAPSD (AA 20-660) protein (His-SUMO Tag)
宿主: Hepatitis E Virus (HEV) 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
CAPSD (AA 1-183), (full length) protein (His tag)宿主: Hepatitis B Virus (HBV) 宿主: 酵母菌 Recombinant > 90 % SDS
CAPSD (AA 1-530), (full length) protein (His tag)宿主: Norwalk virus 宿主: 酵母菌 Recombinant > 90 % SDS
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1-2 mg/mL
-
缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
-
储存条件
- -80 °C,4 °C,-20 °C
-
储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
-
- CAPSD
-
物质类
- Viral Protein
-
背景
- Capsid protein self-assbles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangents leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal mbrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids .
-
分子量
- 64.89 kDa
-
UniProt
- P07299
抗原
-
