Gag (AA 216-478) protein (GST tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Gag (AA 216-478) protein (GST tag) (ABIN5710990)
抗原
Gag
蛋白类型
Recombinant
宿主
Moloney Murine Leukemia Virus (MoMuLV)
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90 %
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产品特性
- AA 216-478
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标记
- GST tag
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序列
- PLRAGGNGQL QYWPFSSSDL YNWKNNNPSF SEDPGKLTAL IESVLITHQP TWDDCQQLLG TLLTGEEKQR VLLEARKAVR GDDGRPTQLP NEVDAAFPLE RPDWDYTTQA GRNHLVHYRQ LLLAGLQNAG RSPTNLAKVK GITQGPNESP SAFLERLKEA YRRYTPYDPE DPGQETNVSM SFIWQSAPDI GRKLERLEDL KNKTLGDLVR EAEKIFNKRE TPEEREERIR RETEEKEERR RTEDEQKEKE RDRRRHREMS KLL
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纯化方法
- SDS-PAGE
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Gag (AA 133-363) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1-2 mg/mL
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缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
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储存条件
- -80 °C,4 °C,-20 °C
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储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Gag
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物质类
- Viral Protein
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背景
- Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.
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分子量
- 58 kDa
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UniProt
- P03332
抗原
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