Crasp-2 蛋白
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- 抗原 See all Crasp-2 products
- Crasp-2 (Complement regulator-acquiring surface protein 2 (Crasp-2))
- 蛋白类型
- Recombinant
- 宿主
- Borrelia burgdorferi
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 000-001-c19
- Supplier
- Rockland
- 原理
- Crasp2 Control Protein
- 纯化方法
- Crasp2 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp2 and was estimated to be greater than 90% pure.
- 过滤
- Sterile filtered
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- 应用备注
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Application Note: Crasp2 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 67.8 kDa for CRASP-2-MBP, (25.4 kDa for CRASP-2 and 42.4 kDa for MBP) and in size corresponding to Crasp2 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 2 was tested in SDS-page and western blot.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- 有效期
- 6 months
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- 抗原
- Crasp-2 (Complement regulator-acquiring surface protein 2 (Crasp-2))
- 别名
- Crasp2 (Crasp-2 产品)
- 背景
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Synonyms: control protein, Borrelia burgdorferi CRASP-2, CRASP2
Background: CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct way. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato has the ability to evade immune systems to persist in a variety of vertebrate hosts. This activity is dependent on a number of factors. Some Borrelia species bind host-derived fluid-phase immune regulators FHL-1 and factor H to their surface via complement regulator-acquiring surface proteins (CRASPs). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby confers resistance of spirochetes to complement-mediated lysis. It is possible that because of discontinuous binding regions in the factor H/FHL-1, long distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may be important in the interaction of B. burgdorferi CRASP-1 with factor H. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
- 基因ID
- 1194149
- NCBI登录号
- WP_010890313
- UniProt
- O50665
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