电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Surface Lipoprotein p27 蛋白

宿主: Borrelia burgdorferi 宿主: 大肠杆菌(E. Coli) Recombinant CP, ELISA, SDS, WB
Rockland
产品编号 ABIN5624579
Supplier Product No.: 000-001-c30
发货至: 中国
  • 抗原
    Surface Lipoprotein p27
    蛋白类型
    Recombinant
    宿主
    Borrelia burgdorferi
    资源
    大肠杆菌(E. Coli)
    应用范围
    Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Supplier Product No.
    000-001-c30
    Supplier
    Rockland
    原理
    Surface Lipoprotein p27 Control Protein
    纯化方法
    Surface Lipoprotein p27 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Surface Lipoprotein p27 and was estimated to be greater than 90% pure.
    过滤
    Sterile filtered
  • 应用备注

    Application Note: Surface Lipoprotein p27 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 73.3 kDa for p27-MBP, (30.9 kDa for p27 and 42.4 kDa for MBP) in size corresponding to Surface Lipoprotein p27 by Western blotting in the appropriate cell lysate or extract. Surface Lipoprotein p27 protein was tested in SDS-page and western blot.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: Lateral Flow Assay: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
    有效期
    6 months
  • 抗原
    Surface Lipoprotein p27
    背景

    Synonyms: BBA060 protein, Borrelia burgdorferi p27, Surface lipoprotein p27, control protein

    Background: Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

    基因ID
    1194336
    NCBI登录号
    WP_010890395
    UniProt
    O50951
You are here:
客服