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- 抗原 See all OspA products
- OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))
- 蛋白类型
- Recombinant
- 宿主
- Borrelia burgdorferi
- 资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Western Blotting (WB)
- 纯化方法
- OspA is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspA and was estimated to be greater than 95% pure.
- 过滤
- Sterile filtered
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- 应用备注
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Application Note: OspA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 70.5 kDa for OspA-MBP, (28.1 kDa for OspA and 42.4 for MBP) in size corresponding to OspA by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.04 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- 有效期
- 6 months
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