Quick Overview for IL23A 蛋白 (ABIN5505700)
抗原
See all IL23A 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-23 alpha / IL23A protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
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Interleukin 23, alpha subunit p19 (IL23A) (AA 1-189) protein (GST tag)
IL23A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 23, alpha subunit p19 (IL23A) (AA 20-157) protein (His-IF2DI Tag)IL23A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) protein (His-Avi Tag,Biotin)IL23A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 23, alpha subunit p19 (IL23A) (AA 22-196) protein (Fc Tag)IL23A 宿主: 小鼠 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 1 x PBS
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
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别名
- Interleukin-23 alpha / IL23A
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分子量
- 55.4 kDa
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NCBI登录号
- NP_112542
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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