IL12A 蛋白
This Recombinant IL12A protein is expressed in Insect Cells.
Quick Overview for IL12A 蛋白 (ABIN5505695)
抗原
See all IL12A 蛋白
IL12A
(Interleukin 12 alpha (IL12A))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Insect Cells
应用范围
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
纯度
95 % , as determined by Coomassie stained SDS-PAGE.
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-12 alpha / IL12A protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.05 - 0.25 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 10^7 units/mg, determined by the production of IFN&gamma by activated human PBMC in response to IL-12.
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Interleukin 12 alpha (IL12A) (AA 144-253) protein (GST tag)
IL12A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL12A (Interleukin 12 alpha (IL12A))
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别名
- Interleukin-12 alpha / IL12A
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背景
- This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
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分子量
- 57 kDa
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NCBI登录号
- NP_000873
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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