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ADIPOQ Protein (His tag)

This Recombinant ADIPOQ protein is expressed in Hi-5 Cells.
产品编号 ABIN5505547
发货至: 中国

Quick Overview for ADIPOQ Protein (His tag) (ABIN5505547)

抗原

See all ADIPOQ 蛋白
ADIPOQ (Adiponectin (ADIPOQ))

蛋白类型

Recombinant

生物活性

Active

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Hi-5 Cells

应用范围

Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

纯度

> 95 % as determined by SDS-PAGE and Coomassie blue staining
  • 标记

    This ADIPOQ protein is labelled with His tag.

    特异性

    Optimal preservation of protein structure, post-translational modifications and functions.

    产品特性

    • Recombinant human Adiponectin protein expressed in Hi-5 Insect cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    内毒素水平

    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

    Biological Activity Comment

    Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 4.0-6.0ug/mL.
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  • 应用备注

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    说明

    The tag is located at the N-terminal.

    限制

    仅限研究用
  • 缓冲液

    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

    注意事项

    Resuspend the protein in the desired concentration in proper buffer

    储存条件

    -80 °C

    储存方法

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • 抗原

    ADIPOQ (Adiponectin (ADIPOQ))

    别名

    Adiponectin

    分子量

    35 kDa

    NCBI登录号

    NP_033735
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