PSI-C core subunit of photosystem I (PsaC) 蛋白
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- 抗原
- PSI-C core subunit of photosystem I (PsaC)
- 宿主
- 请咨询
- 应用范围
- Western Blotting (WB)
- 品牌
- SERVA®
- 纯化方法
- affinity purified
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- 应用备注
- Standard curve: 3 loads are recommended (0.5, 2 and 4i1/4l).For most applications a sample load of 0.2i1/4g of chlorophyll will give a PsaC signal in this range.Positive control: a 2i1/4l load per well is optimal for most chemiluminescent detection systems. This standard is ready-to-load and does not require any additions or heating.Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
- 说明
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Concentration: after adding 225 μl of sterile milliQ water final concentration of the standard is 0.15 pmoles/μlProtein standard buffer composition: Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 225 μL of milliQ water.Please notice that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized.
- 缓冲液
- Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/mL PefaBloc protease inhibitor (Roche), 50 mM DTT.
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- 抗原
- PSI-C core subunit of photosystem I (PsaC)
- 背景
- PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.This product is a recombinant protein standard, source: Synechocystis PCC 6803.
- 分子量
- 11.5 kDa (larger than native protein due to the addition of His-tag). In most gels PsaC migrates between 9 and 14 kDa
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