Western Blotting (WB), ELISA, Immunodiffusion (ID), Immunoelectrophoresis (IEP), Binding Studies (Bind), Dot Blot (DB), Haemagglutination (H)
产品特性
Source: Bovine serum
纯化方法
Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 35 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces of IgM, IgA, traces of complement C3c, transferrin and albumin. Chromatographically purified bovine albumin
Purified bovine albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized, stable white powder.
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.