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Albumin Protein (ALB) (TRITC)

This Albumin protein is expressed in 牛.
产品编号 ABIN457572
发货至: 中国

Quick Overview for Albumin Protein (ALB) (TRITC) (ABIN457572)

抗原

See all Albumin (ALB) 蛋白
Albumin (ALB)

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应用范围

Staining Methods (StM)

纯度

> 90 %
  • 标记

    This Albumin protein is labelled with TRITC.

    产品特性

    Tetramethylrhodamine isothiocyanate-conjugated bovine serum albumin, Cohn fraction V. Source: Pooled normal bovine serum

    纯化方法

    Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains traces transferrin and some alpha-proteins.
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  • 应用备注

    As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the orange-red fluorescence of BSA/TRITC. The purpose of counterstaining is to enhance the optical contrast of the immunospecific staining against a counterstained background.

    限制

    仅限研究用
  • 状态

    Lyophilized

    浓度

    BSA concentration 11.6 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.0.

    缓冲液

    Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2).

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
  • 抗原

    Albumin (ALB)

    别名

    Serum Albumin

    背景

    Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to bovine total serum proteins, to bovine IgA, IgM and albumin

    途径

    Lipid Metabolism
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