IgE 蛋白
Our Local Distributor
北京 101111
Quick Overview for IgE 蛋白 (ABIN363702)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
特异性
- Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
-
产品特性
-
IGHE, chromosome 14q32.33
Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system) -
纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
-
过滤
- Sterile filtered
-
-
IgE (AA 209-428) protein (His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
-
-
应用备注
- For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS, pH=7.2, sterile filtered, contains no NaN3
-
储存液
- Azide free
-
储存条件
- 4 °C
-
-
- IgE
-
物质类
- Antibody
-
背景
- Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.
抗原
-
