IgE 蛋白

ABIN363697 产品详细信息, 供应商: Log in to see
  • IGH2
  • IgE
  • Gm900
  • immunoglobulin heavy chain (epsilon polypeptide)
  • immunoglobulin heavy constant epsilon
  • Immunoglobulin heavy constant epsilon
  • Ighe
  • IGHE

Functional Studies (Func), Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB)
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特异性 Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
产品特性 IGHE, chromosome 14q32.33
Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system)
纯化方法 Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
纯度 > 95 %, tested by analytical Gel Permeation Chromatography
背景 Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.
应用备注 For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
限制 仅限研究用
浓度 1 mg/mL
缓冲液 PBS, pH=7.2, 0.1 % NaN3
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件 4 °C
 image for IgE protein (ABIN363697) product purity: gel permeation chromatography (Superose 12/HR)
 image for IgE protein (ABIN363697) Target binding kinetics (Biacore
 image for IgE protein (ABIN363697) binding of increasing amounts of IgE(SUS-11) to recombinant rat RBL2H3 mast cells exp...
背景 Pachlopnik, Staempfli, Rudolf, Aebischer, Kricek, Miescher, Stadler: "Antigen interaction and heat inactivation expose new epitopes on human IgE." in: International archives of allergy and immunology, Vol. 117, Issue 4, pp. 231-8, 1999 (PubMed).

Zuercher, Lang, Aebischer, Miescher, Stadler: "IgE-producing hybridomas established after B-cell culture in the CD40 system." in: Immunology letters, Vol. 46, Issue 1-2, pp. 49-57, 1995 (PubMed).