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RBM7 Protein (AA 1-265) (Strep Tag)

This Recombinant RBM7 protein is expressed in Cell-free protein synthesis (CFPS).
产品编号 ABIN3128367
发货至: 中国

Quick Overview for RBM7 Protein (AA 1-265) (Strep Tag) (ABIN3128367)

抗原

See all RBM7 蛋白
RBM7 (RNA Binding Motif Protein 7 (RBM7))

蛋白类型

Recombinant

宿主

  • 2
  • 1
  • 1
小鼠

资源

  • 2
  • 1
  • 1
Cell-free protein synthesis (CFPS)

应用范围

ELISA, Western Blotting (WB), SDS-PAGE (SDS)

纯度

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

质量等级

custom-made
  • 产品特性

    AA 1-265

    标记

    This RBM7 protein is labelled with Strep Tag.

    序列

    MGAAAAEADR TLFVGNLETK VTEELLFELF HQAGPVIKVK IPKDKDGKLK QFAFVNFKHE VSVPYAMNLL NGIKLFGRPI KIQFRSGSSH ASQDASVSYP QHHVGNLSPT STSPNSYERT VGNVSPTAQM VQRSFSSPED YQRQAVMNSV FRQMSYAGKF GSPHADQLGF SPSAQPHGHT FNQSSSSQWR QDALSSQRKR QNSHPYLADR HYSREQRYSD HGSDYHYRGS REDFYYDDRN HDGWSHDYDN RRDSSRGGKW PSSRH
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    产品特性

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • 应用备注

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -80 °C

    储存方法

    Store at -80°C.

    有效期

    12 months
  • 抗原

    RBM7 (RNA Binding Motif Protein 7 (RBM7))

    别名

    Rbm7

    背景

    RNA-binding protein 7 (RNA-binding motif protein 7),FUNCTION: RNA-binding subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that functions as an RNA exosome cofactor that directs a subset of non-coding short-lived RNAs for exosomal degradation. NEXT is involved in surveillance and turnover of aberrant transcripts and non-coding RNAs. Binds preferentially polyuridine sequences and associates with newly synthesized RNAs, including pre-mRNAs and short-lived exosome substrates such as promoter upstream transcripts (PROMPTs), enhancer RNAs (eRNAs), and 3'-extended products from small nuclear RNAs (snRNAs). Participates in several biological processes including DNA damage response (DDR) and stress response. During stress response, activation of the p38MAPK-MK2 pathway decreases RBM7-RNA-binding and subsequently the RNA exosome degradation activities, thereby modulating the turnover of non-coding transcriptome. Participates in DNA damage response (DDR), through its interaction with MEPCE and LARP7, the core subunits of 7SK snRNP complex, that release the positive transcription elongation factor b (P-TEFb) complex from the 7SK snRNP. In turn, activation of P-TEFb complex induces the transcription of P-TEFb-dependent DDR genes to promote cell viability. {ECO:0000250|UniProtKB:Q9Y580}.

    分子量

    30.1 kDa

    UniProt

    Q9CQT2
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