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TRIM56 Protein (AA 1-734) (Strep Tag)

This Recombinant TRIM56 protein is expressed in Cell-free protein synthesis (CFPS).
产品编号 ABIN3124317
发货至: 中国

Quick Overview for TRIM56 Protein (AA 1-734) (Strep Tag) (ABIN3124317)

抗原

See all TRIM56 蛋白
TRIM56 (Tripartite Motif Containing 56 (TRIM56))

蛋白类型

Recombinant

宿主

  • 2
  • 1
小鼠

资源

  • 2
  • 1
Cell-free protein synthesis (CFPS)

应用范围

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

纯度

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

质量等级

custom-made
  • 产品特性

    AA 1-734

    标记

    This TRIM56 protein is labelled with Strep Tag.

    序列

    MNSKVSSPTL LEALSSDFLA CKICLEQLHT PKTLPCLHTY CQDCLAQLDI GGQVRCPECR EIVPVPAEGV AAFKTNFFVN GLLDLVKARA PGDVHSGKPT CALCPLVGGK SSGGPATARC LDCADDLCQA CADGHRCSRQ THKHRVVDLV GYRAGWYDEE ARERQASQCP QHPGEALCFL CQPCSQLLCK DCRLGPHIDH PCLPLAEAVR SRKPGLEELL AGVDSNLVEL EATRVAEKEA LALLREQAAS VGTQVEEAAE RILKSLLAQK QEVLGQLRAL VEAAEEATRE RLTKIERQEQ VAKAAAAFAR RVLSLGLEAE ILSLEGAITQ RLRQLQDAPW TSGPTRCVLP QLELHPGLED KNCHLLRLIF EEPKQSPKDS GKGGAGTQGG DEAQGQGDDR TKIGKQGGAQ PLTPKEGKDQ NPQEDDGVFI ERGNRPNKKK KCKGRGKSVS REPSPILRPN LEGSGLLPRP VFSWSFPTRM PGDKRSPRIT GLCPYGPQEI LVADEQNRVL KRFSLNGDYK GTVQVPEGCS PCSVAALQNA VAFSANAKLY LVSPDGEIQW RRSLSLTQSS HAVAAMPCGD RVAVSVAGHV EVYKKDGSLA TRFIPGGKAS RGQRALVFLT TSPQGNFVGS DWQQNSVVFC DGLGQVIWEY KGPGLHGCQP GSVSVDKKGY IFLTLREVNK VVILDPKGSL LGDFLTAYHG LEKPRVTTMV DGKYLVVSLS NGTIHVFRVR FPDS
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    产品特性

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • 应用备注

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -80 °C

    储存方法

    Store at -80°C.

    有效期

    12 months
  • 抗原

    TRIM56 (Tripartite Motif Containing 56 (TRIM56))

    别名

    Trim56

    背景

    E3 ubiquitin-protein ligase TRIM56 (EC 2.3.2.27) (Tripartite motif-containing protein 56),FUNCTION: E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1 (PubMed:21074459, PubMed:29426904). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (PubMed:21074459). Also mediates monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation (PubMed:29426904). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling (By similarity). Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES (By similarity). {ECO:0000250|UniProtKB:Q9BRZ2, ECO:0000269|PubMed:21074459, ECO:0000269|PubMed:29426904}.

    分子量

    79.5 kDa

    UniProt

    Q80VI1
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