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SPOCD1 Protein (AA 1-1216) (Strep Tag)

custom-made SPOCD1 宿主: 人 宿主: Cell-free protein synthesis (CFPS) Recombinant > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
产品编号 ABIN3095621
发货至: 中国
  • 抗原 See all SPOCD1 products
    SPOCD1 (SPOC Domain Containing 1 (SPOCD1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-1216
    宿主
    资源
    • 1
    Cell-free protein synthesis (CFPS)
    标记
    This SPOCD1 protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    品牌
    AliCE®
    序列
    MSQAGDVEGP STGDPVLSPQ HNCELLQNME GASSMPGLSP DGPGASSGPG VRAGSRRKIP RKEALRGGSS RAAGAAEVRP GVLELLAVVQ SRGSMLAPGL HMQLPSVPTQ GRALTSKRLQ VSLCDILDDS CPRKLCSRSA GLPERALACR ERLAGVEEVS CLRPREARDG GMSSPGCDRR SPTLSKEEPP GRPLTSSPDP VPVRVRKKWR RQGAHSECEE GAGDFLWLDQ SPRGDNLLSV GDPPQVADLE SLGGPCRPPS PKDTGSGPGE PGGSGAGCAS GTEKFGYLPA TGDGPQPGSP CGPVGFPVPS GGESLSSAAQ APPQSAALCL GASAQASAEQ QEAVCVVRTG SDEGQAPAQD QEELEAKAQP ASRGRLEQGL AAPADTCASS REPLGGLSSS LDTEASRACS GPFMEQRRSK GTKNLKKGPV PCAQDRGTDR SSDNSHQDRP EEPSPGGCPR LEEVKIPHGV KLVCYLGSGP VIQLLGAISH GQAGGQLPPK LEVLEDLMEV SSPSPAQRLR RKKRPMVQGP AGCQVFQPSP SGGTAGDPGG LSDPFYPPRS GSLALGDPSS DPACSQSGPM EAEEDSLPEQ PEDSAQLQQE KPSLYIGVRG TVVRSMQEVL WTRLRELPDP VLSEEVVEGI AAGIEAALWD LTQGTNGRYK TKYRSLLFNL RDPRNLDLFL KVVHGDVTPY DLVRMSSMQL APQELARWRD QEEKRGLNII EQQQKEPCRL PASKMTHKGE VEIQRDMDQT LTLEDLVGPQ MFMDCSPQAL PIASEDTTGQ HDHHFLDPNC HICKDWEPSN ELLGSFEAAK SCGDNIFQKA LSQTPMPAPE MPKTRELSPT EPQDRVPPSG LHVPAAPTKA LPCLPPWEGV LDMFSIKRFR ARAQLVSGHS CRLVQALPTV IRSAGCIPSN IVWDLLASIC PAKAKDVCVV RLCPHGARDT QNCRLLYSYL NDRQRHGLAS VEHMGMVLLP LPAFQPLPTR LRPLGGPGLW ALPVSPLLSP GLEVTHSSLL LAVLLPKEGL PDTAGSSPWL GKVQKMVSFN SKVEKRYYQP DDRRPNVPLK GTPPPGGAWQ QSQGRGSIAP RGISAWQRPP RGRGRLWPEP ENWQHPGRGQ WPPEPGLRQS QHPYSVAPAG HGFGRGQHFH RDSCPHQALL RHLESLATMS HQLQALLCPQ TKSSIPRPLQ RLSSALAAPE PPGPARDSSL GPTDEAGSEC PFPRKA
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    纯度
    > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    质量等级
    custom-made
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    12 months
  • 抗原
    SPOCD1 (SPOC Domain Containing 1 (SPOCD1))
    别名
    SPOCD1 (SPOCD1 产品)
    背景
    SPOC domain-containing protein 1,FUNCTION: Essential excecutor of PIWIL4-piRNA pathway directed transposon DNA methylation and silencing in the male embryonic germ cells (By similarity). Associates with the de novo DNA methylation machinery and repressive chromatin remodeling complexes (By similarity). Tethering of PIWIL4 to a nascent transposable element transcript recruits repressive chromatin remodeling activities and the de novo methylation apparatus through SPOCD1 (By similarity). Not required for piRNA biosynthesis (By similarity). {ECO:0000250|UniProtKB:B1ASB6}.
    分子量
    130.0 kDa
    UniProt
    Q6ZMY3
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