Streptavidin Protein (HRP)
宿主: Streptomyces avidinii
宿主: Streptomyces avidinii
Native
WB, ELISA, DB
(51 references)-
- 抗原 See all Streptavidin 蛋白
- Streptavidin
- 蛋白类型
- Native
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宿主
- Streptomyces avidinii
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资源
- Streptomyces avidinii
- 标记
- This Streptavidin protein is labelled with HRP.
- 应用范围
- Western Blotting (WB), ELISA, Dot Blot (DB)
- 产品特性
- Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
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Streptavidin protein (HRP)
宿主: Streptomyces avidinii WB, ELISA, IHC, DB, IF
Streptavidin protein (PE)宿主: Streptomyces avidinii FACS, IM
Streptavidin protein (Rhodamine)宿主: Streptomyces avidinii
Streptavidin protein (Atto 425)宿主: Streptomyces avidinii
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- 应用备注
- Dot blot(ECM)|0.25-0.5 μg/mL| Western blot(DAB)|0.3-2 μg/mL| Western blot(ECM)|0.1-0.3 μg/mL| ELISA|0.025-0.05 μg/mL|
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 储存条件
- 4 °C
- 储存方法
- At 4°C for one year.
- 有效期
- 12 months
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: "Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway." in: Oncology reports, Vol. 38, Issue 2, pp. 745-754, (2018) (PubMed).
: "Beneficial effects of extracts from Lucilia sericata maggots on burn wounds in rats." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7213-7220, (2018) (PubMed).
: "The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3455-3464, (2018) (PubMed).
: "Increased expression of hepcidin and associated upregulation of JAK/STAT3 signaling in human gastric cancer." in: Oncology letters, Vol. 15, Issue 2, pp. 2236-2244, (2018) (PubMed).
: "Placenta-specific drug delivery by trophoblast-targeted nanoparticles in mice." in: Theranostics, Vol. 8, Issue 10, pp. 2765-2781, (2018) (PubMed).
: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." in: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).
: "Effects of 1,25-dihydroxyvitamin D3 on the local bone renin-angiotensin system in a murine model of glucocorticoid-induced osteoporosis." in: Experimental and therapeutic medicine, Vol. 13, Issue 6, pp. 3297-3304, (2017) (PubMed).
: "PDCD1 strengthens the sensitivity of ovarian cancer to cisplatin chemotherapy by promoting apoptosis." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 22, Issue 3, pp. 746-756, (2017) (PubMed).
: "The Protective Effect of Salidroside on Hypoxia-Induced Corpus Cavernosum Smooth Muscle Cell Phenotypic Transformation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3530281, (2017) (PubMed).
: "Intercellular adhesion molecule-1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 3391-7, (2015) (PubMed).
: "Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin ..." in: International journal of molecular medicine, Vol. 35, Issue 5, pp. 1199-212, (2015) (PubMed).
: "Pancreatic satellite cells derived galectin-1 increase the progression and less survival of pancreatic ductal adenocarcinoma." in: PLoS ONE, Vol. 9, Issue 3, pp. e90476, (2015) (PubMed).
: "Overexpression of SATB1 is associated with biologic behavior in human renal cell carcinoma." in: PLoS ONE, Vol. 9, Issue 5, pp. e97406, (2015) (PubMed).
: "Overexpression of MERTK receptor tyrosine kinase in epithelial cancer cells drives efferocytosis in a gain-of-function capacity." in: The Journal of biological chemistry, Vol. 289, Issue 37, pp. 25737-49, (2015) (PubMed).
: "Inhibition of Lon blocks cell proliferation, enhances chemosensitivity by promoting apoptosis and decreases cellular bioenergetics of bladder cancer: potential roles of Lon as a prognostic marker and ..." in: Oncotarget, Vol. 5, Issue 22, pp. 11209-24, (2015) (PubMed).
: "Silencing XIAP suppresses osteosarcoma cell growth, and enhances the sensitivity of osteosarcoma cells to doxorubicin and cisplatin." in: Oncology reports, Vol. 33, Issue 3, pp. 1177-84, (2015) (PubMed).
: "Molecular and functional characterization of CD59 from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae." in: Fish & shellfish immunology, Vol. 44, Issue 1, pp. 50-9, (2015) (PubMed).
: "Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats." in: Neural regeneration research, Vol. 7, Issue 6, pp. 421-5, (2015) (PubMed).
: "Diastolic dysfunction in spontaneous type 2 diabetes rhesus monkeys: a study using echocardiography and magnetic resonance imaging." in: BMC cardiovascular disorders, Vol. 15, pp. 59, (2015) (PubMed).
: "Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 995-1002, (2015) (PubMed).
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: "Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway." in: Oncology reports, Vol. 38, Issue 2, pp. 745-754, (2018) (PubMed).
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
- Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
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