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Quick Overview for ZAP70 Protein (His tag) (ABIN3012230)
抗原
See all ZAP70 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This ZAP70 protein is labelled with His tag.
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原理
- Mouse Zeta Chain Associated Protein Kinase 70 kDa (zAP70) Protein
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序列
- A HPSTFTQPQR RVDTLNSDGY TPEPARLASS TDKPRPMPMD TSVYESPYSD PEELKDKKLF LKRENLLVAD IELGCGNFGS VRQGVYRMRK KQIDVAIKVL KQGTEKADKD EMMREAQIMH QLDNPYIVRL IGVCQAEALM LVMEMAGGGP LHKFLLGKKE EIPVSNVAEL LHQVAMGMKY LEEKNFVHRD LAARNVLLVN RHYAKISDFG LSKALGADDS YYTARSAGKW PLKWYAPECI NFRKFSSRSD VWSYGVTMWE AFSYGQKPYK K
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内毒素水平
- < 1.0 EU/μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-493) protein (GST tag)
ZAP70 宿主: 人 宿主: 小麦胚 Recombinant KAA
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 397-493) protein (GST tag)ZAP70 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ZAP70 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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缓冲液
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose and Proclin-300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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别名
- zeta Chain Associated Protein Kinase 70kDa
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分子量
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Calculated MW: 34.7 kDa
Observed MW (SDS-PAGE): 35 kDa, 44 kDa -
UniProt
- P43404
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
-
