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Protease protein (His tag)

This Recombinant Protease protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN3011321
发货至: 中国
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Quick Overview for Protease protein (His tag) (ABIN3011321)

抗原

Protease

蛋白类型

Recombinant

宿主

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资源

  • 6
大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS), Western Blotting (WB)

纯度

> 90 %
  • 标记

    His tag

    原理

    Human Serine Protease 1 (PRSS1) Protein

    序列

    SLINEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPQYD RKTLNNDIML IKLSSRAVIN ARVSTISLPT APPATGTKCL ISGWGNTASS GADYPDELQC LDAPVLSQAK CEASYPGKIT SNMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGVVSWGDGC AQKNKPGV

    Biological Activity Comment

    Not tested
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Please note that the majority of this manufacturers proteins are partial length rather than full length.
    We recommend customers to inquire.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

    缓冲液

    Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 1 mM EDTA, 5 % Trehalose.

    储存液

    Other preservative

    储存条件

    4 °C,-80 °C

    储存方法

    Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    Protease

    物质类

    Viral Protein

    分子量

    Calculated MW: 22.7 kDa
    Observed MW (SDS-PAGE): 26 kDa

    Possible reasons why the actual band size differs from the predicted band size:
    1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

    基因ID

    5644

    HGNC

    9475

    OMIM

    167800

    UniProt

    P07477
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