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Quick Overview for PCK1 Protein (His tag) (ABIN3011147)
抗原
See all PCK1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This PCK1 protein is labelled with His tag.
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原理
- Mouse Phosphoenolpyruvate Carboxykinase, Cytosolic (PCK1) Protein
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序列
- IAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA KLTPIGYIPK ENA
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内毒素水平
- < 1.0 EU per 1 μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Mouse价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Mouse价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)
custom-made PCK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)custom-made PCK1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (His tag)PCK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) protein (GST tag)PCK1 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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缓冲液
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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储存液
- Dithiothreitol (DTT), ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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别名
- Phosphoenolpyruvate Carboxykinase 1
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分子量
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Calculated MW: 31.9 kDa
Observed MW (SDS-PAGE): 27 kDa -
UniProt
- Q9Z2V4
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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